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The present manual, "Molecular Biology and Biotechnology: A Practical Manual for Techniques in Molecular Biology-I", has been developed to provide a structured and comprehensive guide to fundamental laboratory techniques in molecular biology. The field of Molecular Biology and Biotechnology has become an essential component of modern biological sciences, with wide applications in agriculture, medicine, industry, and environmental studies.This manual is designed primarily for postgraduate students and researchers to facilitate a practical understanding of core experimental techniques. It covers a range of foundational methodologies including preparation of reagents, microbial culture techniques, nucleic acid isolation, electrophoresis, PCR, recombinant DNA technology, blotting techniques, and immunological assays. Each experiment has been organised systematically to support effective teaching, learning, and laboratory execution.