Proteomics Mass Spectrometry Methods

Proteomics Mass Spectrometry Methods: Sample Preparation, Protein Digestion, and Research Protocols is the perfect reference for Bioscience researchers entering the realm of Proteomics. With a strong focus on sample preparation, the book share best practices - collected across a network of key laboratories and core facilities - taking the reader all the way to executing the most usual Mass Spectrometry experiments. In part one, the book provides a guide to extract maximum benefit from the book and an overview of proteomics including a review of exciting research making use of MS proteomics experiments. Part two focuses on the critical sample preparation stage. Chapters cover mammalian cell lines, samples from clinical tissue and biological fluids. A further chapter covers subcellular fractionation. The third part provides methods for protein digestion both for in gel and in solution, including specific methods for Shotgun proteomics. The fourth and final part of the book delves into key MS proteomics analysis protocols including label-free LC-MS, TMT and iTRAQ labelled LC-MS, phosphorylation enrichment, ubiquination enrichment, glycosylation enrichment, LC-MS methods for protein identification. The final chapter covers targeted MS using MRM. All chapters include related analytical software as appropriate. Proteomics Mass Spectrometry Methods: Sample Preparation, Protein Digestion, and Research Protocols is the perfect lab manual for Proteomic analysis in Biosciences. It can be used both as a reference for research teams or new staff training material. Core facility managers may find it also useful for sharing best practices with their staff and researchers. Explores the most common questions new researchers in this area have Guides readers to properly design the workflow for successful integration of mass spectrometry into protein biochemical analyses Provides examples of sample preparation for a number of different materials, mammalian cells and others